Structure and Organisation of Interphase Microtubule Arrays: FESEM Provides a MAP
نویسندگان
چکیده
منابع مشابه
A multicomponent assembly pathway contributes to the formation of acentrosomal microtubule arrays in interphase Drosophila cells.
In animal cells, centrosomes nucleate microtubules that form polarized arrays to organize the cytoplasm. Drosophila presents an interesting paradox however, as centrosome-deficient mutant animals develop into viable adults. To understand this discrepancy, we analyzed behaviors of centrosomes and microtubules in Drosophila cells, in culture and in vivo, using a combination of live-cell imaging, ...
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We report the results of studies in which partially purified centrosomes, nuclei, and DNA were injected into frog's eggs, which are naturally arrested in metaphase or interphase. These results have led to an independent assessment of the contributions of the centrosome and the chromatin to the formation of the mitotic spindle and suggest a simple explanation for the transition from interphase t...
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The extent and pattern of the rearrangements of microtubule arrays in interphase and mitotic PtK2 cells treated with deuterium oxide (2H2O) were evaluated using light, immunofluorescence and electron microscopy. Combined labelling with anti-tubulin antibodies and staining with a DNA-specific fluorochrome revealed that 2H2O influences the reassembly of the cytoplasmic microtubule complex (CMTC) ...
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Cytoplasmic dynein is a microtubule-based motor protein responsible for vesicle movement and spindle orientation in eukaryotic cells. We show here that dynein also supports microtubule architecture and determines centrosome position in interphase cells. Overexpression of the motor domain in Dictyostelium leads to a collapse of the interphase microtubule array, forming loose bundles that often e...
متن کاملMicrotubule dynamics in interphase cells
The sites of microtubule growth and the kinetics of elongation have been studied in vivo by microinjection of biotin-labeled tubulin and subsequent visualization with immunocytochemical probes. Immunofluorescence and immunoelectron microscopy demonstrate that injected biotin-labeled subunits are incorporated into new segments of growth which are contiguous with unlabeled microtubules. Rapid inc...
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ژورنال
عنوان ژورنال: Microscopy and Microanalysis
سال: 2006
ISSN: 1431-9276,1435-8115
DOI: 10.1017/s1431927606069819